SPAOM 2018 – 23/10/2018
Granada (Spain), 23-26 October 2018
VERTEX Technics estará presente en la próxima edición del REMOA que tendrá lugar en Granada.
Estaremos presentes con un stand donde podrá ver en funcionamiento el nuevo ImageXpress pico y también realizaremos dos symposiums científicos impartidos por:
Penny Tavormina, PhD, Application Scientist
- Morphological and Functional Evaluation of Compound Activity in 3D Cultures Using High Content Screening.
There is an increasing interest in using more complex, biologically relevant, and predictive cell-based assays for assay development and compound screening. We will present multiparametric data comparing 2D and 3D cell culture models in automated confocal imaging of neurotoxicity and hepatotoxicity assays. Data presented include concentration-dependent inhibitory effects of compounds on the complexity of 3D networks. We will discuss functional and morphological assays used to evaluate 3D spheroids and organoids supporting their use in testing drug candidates and for toxicity assessment.
- Quantitative Image Analysis of Cytotoxicity using Stem Cell Models.
Human iPSC-derived neurons, cardiomyocytes and hepatocytes were treated with compounds and evaluated using automated image acquisition and analysis. The iPSC-derived cells are a valuable cell model which closely mimic the phenotypes of primary cells while reducing some of the challenges with working with primary cells. We have used both transmitted light and fluorescent automated microscopy to examine viability and phenotypic changes in stem cell models. We will present quantitative multi parametric data enabling discrimination between different cell populations and compounds.