4th REMOA Conference

Hace un par de semanas VERTEX Technics estuvo en la 4th REMOA Conference. Presentando sus novedades junto a Molecular Devices.

Allí contamos con la presencia de un equipo ImageXpress Pico  y con el soporte de Penny Tavormina, PhD (Application Scientist).  Quien realizó dos presentaciones muy interesantes:

 

1. Morphological and Functional Evaluation of Compound Activity in 3D Cultures Using High Content Screening.

There is an increasing interest in using more complex, biologically relevant, and predictive, cell-based assays for assay development and compound screening. We will present multiparametric data comparing 2D and 3D cell culture models. In automated confocal imaging of neurotoxicity and hepatotoxicity assays.  Data presented include concentration-dependent inhibitory effects of compounds on the complexity of 3D networks. We will discuss functional and morphological assays, used to evaluate 3D spheroids and organoids, supporting their use in testing drug candidates and for toxicity assessment. 

 

2. Quantitative Image Analysis of Cytotoxicity using Stem Cell Models.

Human iPSC-derived neurons, cardiomyocytes and hepatocytes were treated with compounds and evaluated using automated image acquisition and analysis.   The iPSC-derived cells are a valuable cell model which closely mimic the phenotypes of primary cells while reducing some of the challenges with working with primary cells.  We have used both transmitted light and fluorescent automated microscopy to examine viability and phenotypic changes in stem cell models.  We will present quantitative multi parametric data enabling discrimination between different cell populations and compounds.   

 

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